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KMID : 1143420160090360712
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Public Health Weekly Report
2016 Volume.9 No. 36 p.712 ~ p.715
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Genetic diversity of Vibiro parahaemolyticus strains isolated in Korea
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Na Hae-Young
Hong Kyu-Rak
Kwak Hyo-Sun
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Abstract
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Background: Vibrio parahaemolyticus is one of the leading causes of food-borne outbreaks and acute gastroenteritis worldwide. The genetic structure of V. parahaemolyticus isolates spreading in Korea remains undefined.
Methodology: In this study, 94 clinical isolates were used to examine the genetic relationship and diversity in Korea during 3 years (2013-2015). Multilocus sequence typing (MLST) method was used to study prevalence and clonal structure of clinical isolated strains. PCR conditions of the seven house keeping genes and sequence types(ST) designation of all isolates were followed as described in pubMLST. A real-time PCR assay was performed to determine the presence of tdh and trh gene. Genetic diversity analysis were carried out using the MEGA 6 software. Based on the related STs, the isolates were subdivided into clonal complex(CC) or groups by goeBURST analysis using PHYLOViZ software.
Results: The 94 tested isolates fell into 11 STs, of which two STs were new to the MLST database. The prevalence of STs was ST-3(38.3%), ST-332(28.7%), ST-8(16.0%), ST-50(7.4%), ST-610(2.1%), 624(2.1%) and the rest of the STs were represented by a single strain. ST-3 was the only ST with an international distribution. The nucleotide diversity ranged from 0.010 (gyrB) to 0.026(dtdS) and recA presented the largest number of variable sites (6.0%, 44/729). goeBURST algorithm analysis showed that tested strains were not so much related to each other, 66 STs out of V. parahaemolyticus pubMLST databaes were formed 5 CCs, 3 groups, and 3 singletons with our 11 STs.
Conclusion: 11 STs were not clustered by region distributions in Korea, but formed 5 CCs, 3 groups, and 3 singletons with pubMLST database. From these, genetic structure of V. parahaemolyticus in Korea seem to not so much different to other countries, suggesting genetic diversity.
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KEYWORD
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Vibrio parahaemolyticus, Multi Locus Sequence Typing (MLST)
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